Journal: The Prostate
Article Title: Altered Glycosylation of PSA in Prostate Cancer Tissue
doi: 10.1002/pros.70014
Figure Lengend Snippet: Examples of total PSA (Mab 5C7, figure panels Bg1 and Ca1) and lectin reactive PSA [PLA with Fab fragment of Mab 5C7 and lectins WGA succ (Bg2 and Ca2), VVA (Bg3 and Ca3) and WGA (Bg4 and Ca4)] in prostate cancer (Ca) and benign (Bg) prostatic tissues (same tissues in all). Inset (Bg1b) in total PSA staining of benign tissue is a negative control with nonimmune IgG. Inset (Ca2b) in PLA staining of cancer tissue is PLA staining in which SJA lectin was used instead of WGA succ . Scale bar (in Bg2) is 250 µm.
Article Snippet: Other lectins were from Vector Laboratories as Biotinylated Lectin Kits I, II and III, or biotinylated MAL I (B‐1315‐2), SNA (B‐1305‐2), and AAL (B‐1395‐1) were obtained separately (Table ).
Techniques: Staining, Negative Control